You are studying a protein that you think functions as a protease. You know the genetic code and amino acid sequence for the protein and you hypothesize that a serine is critical to the enzyme's function. Describe an experiment or set of experiments that would test your hypothesis.

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21-02-2022
Biology

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You are studying a protein that you think functions as a protease. You know the genetic code and amino acid sequence for the protein and you hypothesize that a serine is critical to the enzyme's function. Describe an experiment or set of experiments that would test your hypothesis.

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luisvaschetto luisvaschetto · Answer 1 · 2022-03-01T14:09:38+01:00

It is possible to remove serine by targeting mutagenesis and then observe how this modification changes the function of the protein in the cell. Serine is often a target for phosphorylation.

What is serine?

Serine is one fundamental amino acid, i.e., a building block of biomolecules called proteins.

Serine is often a target for posttranslational modifications, especially protein phosphorylation.

In a given cell, serine phosphorylation may activate or deactivate specific signaling pathways.

In this case, it is possible to identify other serine proteases by sequence homo-logy using the BLAST bioinformatic tool.

Learn more about serine proteases here:

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