The group that will most probably detect the bitter substance the most would be those drinking first.
When the bitter substance was added, it wasn't stirred. This means that for it to be completely diluted, diffusion must take place.
While this is taking place however, the part that the bitter substance first came in contact with, will be more bitter. This is the part that those who drink first will take from and so they will detect it the bitter substance more.
Find out more on diffusion at https://brainly.com/question/8880115.