Question

43. A stock glucose standard has a concentration of 1,000 mg/dL. A 1/5 dilution of this standard is made. What would be the final concentration of the solution

Answer 1

The final concentration of the diluted standard is 0.2 mg/dL.

What is concentration of glucose standard after 1/5 solution?

Using the dilution formula:

• C1V1 = C2V2

where

• C1 is initial concentration
• V1 initial volume
• C2 is final concentration
• V2 is final volume.

Assuming a final volume of 100 mL, and since a 1/5 dilution is made:

C1 = 1.00 mg/dL

V1 = 20

C2 = ?

V2 = 100 mL

C2 = C1V1/V2

C2 = 20 × 1/100

C2 = 0.2 mg/dL

Therefore, the final concentration of the diluted standard is 0.2 mg/dL.

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