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Answer: DNA sequencing methods employ chain termination nucleotides. Furthermore, if two chain-terminating nucleotides are employed in the same well, determining which strand is ended by which dideoxynucleotide will be impossible.
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- Chain termination nucleotides are used in DNA sequencing techniques.
- Furthermore, it will be impossible to tell which strand is terminated by which dideoxynucleotide if two chain-terminating nucleotides are used in the same well.
Why is it necessary to include only one chain terminating synthesis terminating nucleotide?
- Only a limited subset of molecules will randomly incorporate dideoxynucleotides when the terminating nucleotide is added in small amounts.
- All permutations of termination can be achieved because different chain-terminating nucleotides are utilized in the four sequencing procedures.
What is a synthesis terminating nucleotide?
- Synthesis comes to an end when a ddNTP is added to a chain of nucleotides.
- This is due to the ddNTP molecule's absence of the 3' hydroxyl group needed to bind to the subsequent nucleotide in the chain.
What is chain termination method of DNA sequencing?
- The "chain termination method," often known as Sanger sequencing, is a technique for figuring out the nucleotide sequence of DNA.
- The Sanger Sequence is a method that was created in 1977 by two-time Nobel laureate Frederick Sanger and his colleagues.
What is the purpose of terminating the replicated strands?
- When two replication forks with opposing polarities come together and combine to form two independent, fully formed double-stranded DNA molecules, DNA replication is stopped.
Learn more about chain-terminating here:
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