Question

what are some important factors to consider when setting up your pcr reactions?

Answer 1

Answer:

• Factors Affecting the PCR:

• Denaturing Temperature and Time: ...

• Annealing Temperature and Primer Design: ...
• Primer Length: ...
• Degenerate Primers: ...
• Elongation Temperature and Time: ...
• PCR Reaction Buffer: ...
• Cycle Number: ...
• Helix De-stabilisers / Additives:

Explanation:

Hope this helped!