3. Given the DNA sequence -- 5’ GGGGCTGTACTATCATGCGTCGTCTCGGUUAAUUU 3’ determine the amino acid sequence of the protein once the gene is expressed. (N.B. Answer must identify the N- terminus and C-terminus.)

Transcription is the process in which RNA nucleotides find their complementary base on the DNA and are joined together to make a single-stranded RNA molecule.

DNA Base RNA Base

C G

G C

T A

A U

Translation - The RNA molecule, generated called messenger RNA or mRNA acts as a messenger to decide which amino acid needs to be added to the peptide chain . The mRNA contains three-base sequences called codons, which tell the ribosome what specific amino acid needs to be added.

Thus,

DNA 5’ GGGGCTGTACTATCATGCGTCGTCTCGGUUAAUUU 3’

mRNA 3' CCC-CGA-CAU-GAU-AGU-ACG-CAG-CAG-AGC-CAA-UUA-AA 5'

protein N-Proline-Arginine-Histidine-Aspartic acid-Serine-Threonine-Glutamine-Glutamine-Serine-Glutamine-Leucine-C

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