Question

In addition to DNA polymerase and primers, the polymerase chain reaction also requires, A. a large amount of DNA, B. restriction enzymes, C. a supply of the four DNA nucleotides, D. complementary sequences of DNA

Answer 1

The answer isC. a supply of the four DNA nucleotides

PCR or polymerase chain reaction is used to amplify the desired DNA sequence into million copies. DNA polymerase, primers, a supply of the four DNA nucleotides. A large amount of DNA is not necessary since we will amplify it in the PCR. Restriction enzymes would cut DNA, and complementary DNA sequence would bind to DNA and disable its amplification.