Question

How does the spectrophotometer measure absorbance? Why does the dye absorb light (electron transitions)? What color light does Blue #1 dye absorb? What is the lambda max of the dye? What is Beer's law and how can it be used to calculate the desired concentrations for your solutions? Considering Beer's law, is it possible to look up the molar absorptivity coefficient of Blue #1 dye? How will you estimate what concentrations of dye will be required for an absorbance of 1.0 AU given a path length of 1.46cm? How would you dilute a 2.0 mM dye solution to make 100mL of this 1.0 AU solution? How can the 1.0 AU solution be diluted to form a point on the calibration curve at 0.25 AU?

Answer 1

Answer:

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Explanation:

Answer 2

The spectroscopy has been an analytical technique used for the qualitative and quantitative determination of the sample.

• The measurement of absorbance by the sample has been given by the amount of light transmitted from the sample in the path of the radiations.

• The dye has been consistent of the atomic particles, the energy has been gained by the atoms for the excitation from the ground state to the excited state.

• The lambda max of a dye has been the wavelength at which the dye has been showing maximum absorbance.

• Beer's law has been that the absorbance of the sample has been directly proportional to the concentration of the sample. The concentration from Beer's law has been given by:

$A=\epsilon \;cl$

Where A= absorbance

The molar absorptivity, $\epsilon$

The concentration of the sample, c

The path length of the sample, l

• The molar absorptivity of the blue dye has been calculated with the known absorbance and concentration.

The absorbance of the sample has been given as:

A=cl

Where, the absorbance of sample, A=1AU

The path length of the sample, l=.46 cm

The concentration of sample has been given by substituting the values as:

$1=1.46\;\times\;c\\c=\dfrac{1}{1.46} \text{M}\\c=0.68\;\text M$

The concentration of the sample with molar absorptivity 1 has been 0.68 M.

• The sample has been diluted to 100 ml with the dilution series. The series has been plotted for the absorbance measurement. The sample with absorbance 1AU has been selected.

• The sample has been diluted in the series of orders and the dilution with the absorbance reading of 0.25 has been selected.

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