Answer:
The correct answer is option E, that is, synthesis of ATP could be mediated by a proton gradient.
Explanation:
Andre Jagendorf inserted chloroplasts for many hours within a buffer of pH 4 and then instantaneously mixed that with the buffer of pH 8. He came to known that in the stroma, a change in pH was witnessed, that is, it moved to 8 while in the thylakoid space there was no change, that is, the pH remained at 4. He then isolated the thylakoid and put it in the dark, he noticed that it produced ATP. This proves the hypothesis of Peter Mitchell that the production of ATP is mediated by proton-motive force.