Answers:
Increased friction with the tribes - Much of the rail lines passed through/close to native lands
Less land for the tribes - Their lands were seized and they were depoted to reservations
Why It's Not:
Increase in the number of slaves entering the territory - Most slavery was in the South, not the West, which was already established as states
Increase in the number of settlers leaving the territory - The purpose of the acts was to make immigration into the territories easier and more efficient because of the large influx of settlers moving into the territories