Lysosomes contain hydrolytic enzymes that derive from the endoplasmic reticulum (ER) and work best under a pH that is different from cytoplasmic pH. These enzymes, called lysozymes, use this low pH to degrade damaged macromolecules, but they are able to spare their own protein and enzyme contents.

Which of the following conditions is likely to cause a buildup of materials in the lysosome?

inhibiting the protein synthesis in the ER
decreasing the supply of amino acids to the cell by half
providing the cell with more nutrients so that more protein can be made
having a mutation in a lysosomal proteinase that renders it more resistant to self-digestion