Question

Which of the statements best describes the way you would engineer bacterial cells to produce a human protein?A. One restriction enzyme would be used to cut the donor human DNA and a different one would be used to cleave the vector DNA. B. One restriction enzyme would be used only to cleave the vector DNA. C. One restriction enzyme would be used only to cleave the donor human DNA. D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA

Answer 1

Answer:

The correct answer is option D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA.

Explanation:

The way one could engineer bacterial cells to produce a human protein is by allowing bacteria to taking up the recombinant DNA. This recombinant DNA are inserted in the form of the vectors.

Recombinant DNA technology  or RDT allows individual fragments of DNA from any donor human genome to be inserted into vector DNA molecules with the help of the specific restriction enzyme for both vector DNA and donor DNA.

Thus, the correct answer is option D. The same restriction enzyme would be used to cut both the donor human DNA and the vector DNA.

Answer 2

The same restriction enzyme would be used to cut both the donor human

DNA and the vector DNA.

Restriction enzymes are also referred to as endonucleases and this involves

the cutting of a sequence at a particular end on the human and donor DNA

for various purposes such as modification etc.

The same restriction enzyme would be used to cut both the donor human

DNA and the vector DNA because it results in complementary sticky ends

being produced which is vital in DNA formation.

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