Question

Determine the correct sequence of events during the creation of recombinant DNA. I. Plasmid is cut open with enzymes II. Target gene is cut out with enzymes III. Plasmid is inserted into the bacterium IV. Target gene is bonded to the plasmid with Ligase

A. IV, III, I, II
B. II, I, IV, III
C. IV, I, II, III
D. I, II, III, IV

Answer 1

Answer:

The correct answer should be choice B if I'm going about this the right way.

Answer 2

The correct sequence is as follows:

II. Target gene is cut out with enzymes

I. Plasmid is cut open with enzymes

IV. Target gene is bonded to the plasmid with Ligase

III. Plasmid is inserted into the bacterium

• Genetic engineering, also known as genetic manipulation, is the use of recombinant DNA to change the genetic makeup of organisms. In the creation of a recombinant DNA, which is usually a plasmid + desired gene, the following processes are employed:

1. The target or desired gene is initially cut out using enzymes called restriction enzymes.

2. The plasmid (extrachromosomal DNA) from a bacterium is then cut open.

3. Ligase enzyme is used to bond the target gene or gene of interest with the plasmid.

4. After bonding, the plasmid is inserted into a bacterium to multiply.

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