A technologist decides to make a 1:20 dilution of cerebrospinal fluid (CSF) after briefly evaluating a portion of the sample microscopically. After making the dilution and charging the chambers, the number of cells that are observed in each of the large squares of the hemocytometer is >100. What should the technologist do to obtain the most accurate count

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Answer:

The technologist needs to obtain a larger dilution.

Explanation:

A hemocytometer is a tool used in the laboratory for counting cells in suspension, it consists of a microscope slide that has a rectangular indentation that forms a chamber where the cells are stably maintained and counted.

The cerebrospinal fluid (CSF) is a liquid that is localized around the brain and spinal cord. Moreover, CSF analysis is a set of laboratory tests to measure the number of red and white blood cells.  

The CSF tests are useful to detect different health problems that affect the central nervous system including, among others, infections in the brain (encephalitis, meningitis), inflammations, tumors, multiple sclerosis, etc.

In a CSF test, the number of white blood cells should be very low, and relatively high concentrations of such cells may be indicative of multiple sclerosis, encephalitis, leukemia, hemorrhage and allergy.

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