Which of the following describes why a drug may appear effective against a pathogen in clinical susceptibility tests but ineffective in a real-life context? To be marked correct, you'll need to select all applicable statements, as there may be more than one correct answer.
1. Compared to controlled susceptibility tests, the drug may be less active in a real-life context either due to drug inactivation by body processes or as a result of drug solubility issues.
2. Drug susceptibility tests are typically performed at room temperature (much cooler than human body temperature).
3. In clinical susceptibility tests, drug concentration is well controlled compared to a real-life context.
4. Unlike in controlled clinical susceptibility tests, the number of bacteria varies between tissue samples.
