Question

A mutation of the proteolytic enzyme Trypsin (described in Section 6.1) results in a stable covalent bond between one of the catalytic amino acids of the protease and its protein substrate. Which of the following are probably true?
a. The enzyme would become inactive.
b. The enzyme and substrate would be stuck together.
c. The delta-G of the proteolysis reaction would change.
d. The rate of the catalyzed reaction would increase.

Answer 1

Answer:

b. The enzyme and substrate would be stuck together.

Explanation:

Enzymes are proteins whose active site binds to specific chemical reactants (i.e., substrates), thereby forming a complex that is similar to the interaction between a lock and its key. This active complex lowers the energy of the reaction and promotes a conformational change in the substrate to break down it into multiple products. When the enzyme contains mutations in its active site, the ability to bind the substrate is altered. In this case, the enzymatic reaction can't occur because the interaction enzyme-substrate doesn't produce an active complex.

Answer 2

In the process of mutation of a proteolytic enzyme, the enzyme and the substrate would be stuck together.

What is the process of mutation of a proteolytic enzyme?

The process of mutation of a proteolytic enzyme is a ubiquitous and permanent post-translational alteration that involves the restricted and highly selective hydrolysis of a protein's peptide and isopeptide bonds by a protease.

We know that enzymes are proteins that have an activation site that binds to certain chemical reactant molecules (e.g substrates), generating a complex akin to the interaction of a lock and its key.

This active complex decreases the reaction's energy and induces a conformational shift in the chemical reactant molecule (substrate), therefore allowing it to be broken down into numerous products.

The capacity of the enzyme to interact with the substrate is changed and altered when it has mutations in its activation site.

Therefore, this enzymatic reaction cannot take place in this scenario due to the fact that the enzyme-substrate contact does not form an active complex.

Learn more about the mutation of the proteolytic enzyme here:

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