Question

How can you determine the optimum pH of an enzyme?

Answer 1

The optimal pH can be measured by looking at enzyme activity verses pH. Enzyme activity will increase up to a certain pH; above that pH, the enzyme activity will begin to fall.

Answer 2

You can determine the optimum pH of an enzyme by graphing its pH activity and studying the results.

Enzymes each perform differently in distinct environments. Some require lower pH to function, while others achieve maximum performance at higher pH levels.  

This relationship between the productivity of the enzyme and the pH can be studied and graphed. Once done, we can expect this graph to look like a parabola, increasing steadily until maxed, then gradually decreasing back to zero at the other extreme. The vertice of this parabola will give us the optimum pH for the enzyme.

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