Question

A researcher is interested in performing indirect immunofluorescence microscopy in human tissue using an antibody raised in goats that can detect a human protein called PCNT. Which of the following antibodies would better work as the secondary antibody in this experiment? Goat anti-goat linked to Cy3 Rat anti-goat linked to Cy3 Human anti-human linked to Cy3 Goat anti-human linked to Cy3

Answer 1

The  better work as the secondary antibody in this experiment is $at-goat$ linked to $cy3.$

Information regarding microscopy:

• In the case of performing an indirect microscopy in human tissue by using of Rat-goat that linked to $cy3$secondary antibody to detect a human protein
• The symbol for the human ge-ne, is $Pericentrin.$
• Ortho-logs for this ge-ne are also found in  Rat

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