Question

You have made random-sequence synthetic RNA molecules that contains only A and G, and which contain 5 times as much A as G. You use these RNAs to perform in vitro translation. How many different amino acids would you expect to find in the resulting polypeptides? And at what frequency would you expect to find Glutamic acid (Glu) in the resulting polypeptides?

Answer 1

Answer:

According to the genetic code, the amino acids are the following:  

- Glycine: encoded by GGC, GGA and GGG codons  

-Arginine: encoded by AGA and AGG codons

-Lysine: encoded by AAA and AAG codons

-Glutamic acid: encoded by GAA and GAG  codons

Explanation:

The probabilities to observe an amino-acid formed by Glutamic acid in the coding sequence is 2/9 for each codon

Answer 2

The resulting polypeptide will contains Glycine, arginine ,lysine , and glutamic acid. The probabilities to form a glutamic acid in the coding sequence is 2/9 for each codon.

Genetic code:

It is a three letter code of base pairs in the nucleic acid that code for an specific amino acid.

Here, mRNA is made up of only A and G. Nine code are possible in a mRNA sequence that contains only A and G.

 

• GGC, GGA and GGG  code for Glycine.  

• AGA and AGG code for Arginine.

• AAA and AAG  code for Lysine.

• GAA and GAG  code for Glutamic acid.  

 

Therefore, the resulting polypeptide will contains Glycine, arginine ,lysine , and glutamic acid. The probabilities to form a glutamic acid in the coding sequence is 2/9 for each codon.

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