The final pattern you wish to obtain expresses the UASG-GFP construct only in cell C2. How will you drive UASG-GFP expression specifically in cell C2? For each regulatory DNA construct, decide if it should express Gal4, Gal80, or not be used in order to produce the desired pattern. Drag the labels on the left to the appropriate blanks on the right to complete the sentences. Labels may be used once, more than once, or not at all. ResetHelp express Gal 4 express Gal 80 not be used 1. Construct 1 should . 2. Construct 2 should . 3. Construct 3 should . 4. Construct 4 should . SubmitRequest Answer Provide Feedback Next

Here, cell C-2 needs to express GFP and therefore, GAL4 should be functional in cell C-2. This can be achieved by expressing GAL4 in Regulatory DNA construct 3, and expressing GAL80, an inhibitor of GAL4 in Regulatory constructs 1 and 2. This arrangement expresses GAL4 in columns A and C, and inhibits GAL4 due to GAL80 expression in Columns A and B; and in rows 1 and 3.

Therefore, the only cell that expresses GAL4 is cell C-2.

The arrangement is:

1. Construct 1 should express GAL80

2. Construct 2 should express GAL80

3. Construct 3 should express GAL4

4. Construct 4 should not be used