Question

You were recently hired by a biotech company to test out their new restriction enzyme. This enzyme recognizes the following sequence: GGATCC. It makes a cut between the two Gs. You have a linear test sample of DNA to try out this new product: 5'-GATCAGCTGACTGGATCCGTCCTCAACGTCAGGATCCAGCTTCAAG-3' Question 3 (2 points) How many cuts do you expect this enzyme to make on the above DNA? Please enter a number. Question 4 (1 point) How many fragments do you expect to see on your gel? Assume that the are all different sizes.

Answer 1

Answer:

The enzyme will make 2 cuts producing three fragnments.

Explanation:

Some enzymes called endonucleases cleave the phosphodiester bonds that exist in the DNA. Some cleave with the aid of recognition sites and are called restriction endonucleases while some cleave non-specifically.

 5'-GATCAGCTGACTGGATCCGTCCTCAACGTCAGGATCCAGCTTCAAG-3'.

The enzyme recognizes the sequence: GGATCC. and makes cuts between the two GG.

In the sequence, there are 2 of GGATCC sequence sites. thus the enzyme will make 2 cuts producing three fragments.

5'-GATCAGCTGACTG GATCCGTCCTCAACGTCAG GATCCAGCTTCAAG-3'