You're the manager of a factory that produces enzyme-washed blue jeans (the enzymes lighten the color of the denim, giving a "faded" appearance). When the most recent batch of fabric came out of the enzyme wash, however, the color wasn't light enough to meet your standards. Your quality control laboratory wants to do some tests to determine why the wash enzymes didn't perform as expected. Which of the following experiments would be best for testing the hypothesis that the enzymes did not perform as expected because the temperature was too high?

A) Produce one batch of blue jeans with twice as much enzyme as another batch of blue jeans while keeping the temperature the same, then compare how the lightness of the blue jeans varies between batches
B) Produce multiple batches of blue jeans at several different temperatures but with the same enzyme concentration and then compare how the lightness of the blue jeans varies between batches
C) Produce multiple batches of blue jeans at several different temperatures and with different enzyme concentrations and then compare how the lightness of the blue jeans varies between batches
D) Produce one batch of blue jeans at a high temperature without the enzyme and another batch of blue jeans at a low temperature with the enzyme and then compare how the lightness of the blue jeans varies between batches

B) Produce multiple batches of blue jeans at several different temperatures but with the same enzyme concentration and then compare how the lightness of the blue jeans varies between batches

Explanation:

Since the experiment is centered on finding out the effect temperature has on the performance of the enzyme,

The best experiment to test the hypothesis that the enzymes did not perform as expected because the temperature was too high is the experiment stated in option B.

Since we are interested in the effect of temperature on the performance of the enzyme, we do need to vary the concentration. Also, to eliminate chances of error, we need to use multiple batches of the jeans produce at different temperature, then later compare the difference in lightness of the blue jeans between different batches produced at different temperatures.