Question

Arrange the following steps from a typical genetic modification procedure into their correct sequence. Place the first step at the far left. Some steps my be equivalent. Rank the typical genetic modification procedure from the first step to the last step. To rank items as equivalent, overlap them.
1) Select the gene of interest + insert into plasmid
2) Protein product harvested from bacteria
3) Isolate bacterial plasmid
4) Bacteria replicate
5a) Cloned gene harvested from bacteria
5b) Plasmid taken up by bacterium

Answer 1

Answer:

The correct order is

1. Isolate bacterial plasmid

2. Select gene of interest & insert into plasmid.

3. Plasmid taken up by bacterium.

4. Bacteria replicate

5.  Cloned gene harvested from bacteria

6. Protein product harvested from bacteria.

Explanation:

In the process of genetic mutation, a gene responsible for any specific trait of with in an organism is extracted in cultivated in a bacterial cell for commercial use.  

In this process, the steps adopted are as follows –  

a) Identify the gene  

b) Isolate the gene

c) Enter the gene into bacterial plasmid

d) Bacteria is allowed to replicate

e) After the replication, cloned gene is harvested

Hence, the correct order is

1. Isolate bacterial plasmid

2. Select gene of interest & insert into plasmid.

3. Plasmid taken up by bacterium.

4. Bacteria replicate

5.  Cloned gene harvested from bacteria

6. Protein product harvested from bacteria.