PCR is a technique used which is used to amplify DNA so, many copies of a DNA fragment will get as a end result of using PCR.
What is DNA?
- DNA stands for deoxyribose nucleic acid.
- It is a self-replicating substance that makes up the majority of chromosomes in practically all living creatures.
- It carries genetic information of living beings.
What is PCR polymerase chain reaction?
- Kary B. Mullis, an American scientist, invented PCR in 1983.
- The polymerase chain reaction (PCR) is a laboratory technique used for rapidly amplifying millions to billions of copies of a single DNA region.
- PCR entails selecting a portion of the genome to be amplified using short synthetic DNA fragments called primers, followed by several rounds of DNA synthesis to amplify that segment.
Amplification is accomplished by a three-step process:-
- Denaturation is the process of heating double-stranded DNA templates to separate the strands at 95°C.
- Annealing is the process of short DNA molecules called primers binding to flanking regions of target DNA at 55°C.
- Extension is the process of DNA polymerase extending the 3′ end of each primer along the template strands at 72°C.
These processes are performed 25–35 times to make precise copies of the target DNA exponentially.
Hence, the correct option is D.
To know more about PCR polymerase chain reaction here
https://brainly.com/question/10388677
#SPJ2