Question

You have a 20kb plasmid. EcoRI cuts the plasmid at the 2kb point and at the 18kb point, while BamHI cuts the plasmid at the 5kb point and the 15kb point. What band pattern would you see on a gel when you cut the plasmid with both restriction enzymes at the same time? (hint: draw it!)

Answer 1

The band pattern will be 3Kb, 4 Kb and 10 Kb on the gel when you cut the plasmid with both restriction enzymes at the same time.

Explanation:

Plasmid genome = 20 Kb

Eco R1 cuts at = 2 Kb point and 18 kb point

Bam H1 cuts at 5 Kb point and 15 kb point

Bam H1 recognizes 6 base pairs and cleaves them

Eco R1 recognizes the target and produces 4 nucleotide stick end having 5' overhang of AATT.

after digestion with these enzymes the bands are seen at 3kb, 4kb and 10 kb.

Digestion with eco R1 creates a band of 4 kb

while digestion with Bam H1 creates two bands one of 3kb and other of 10 kb.

Figure attached to show the cuts in plasmid by EcoR1 and Bam H1.