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Which of the following had the greatest impact on the institution of slavery in the United States in the first quarter of the nineteenth century

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Answer:

In 1820, the Missouri Compromise had attempted to resolve this question: It had admitted Missouri to the union as a slave state and Maine as a free state, preserving the fragile balance in Congress. More important, it had stipulated that in the future, slavery would be prohibited north of the southern boundary of Missouri (the 36 º 30’ parallel) in the rest of the Louisiana Purchase.

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