Answer:
4 chemicals
Explanation:
There are mainly 4 steps of gram staining.
1- crystal violet stain drained on the heat fixed slide.
2- then wash the slide and use iodine.
3- then wash the slide with water and use acetone as a decolorizer.
4- then use safranine as a counter stain.
So there are a total of 4 chemicals used named crystal violet, iodine, acetone, and safranine.
