Question

True or false?

Suppose you have a PCR amplicon consisting of the sequence below. The primers used to generate the amplicon are: 5'AT3' and 5'GA3'. You can use one of these primer sequences for a subsequent sequencing reaction but not both simultaneously because they will generate sequence information for different nucleotide chains. The chains are complementary but not identical therefore fragments of the same size will be likely to contain different chain terminating nucleotides. For example, 4 base long sequence fragments using 5'AT3' will terminate in a G, whereas 4 base long sequence fragments using 5'GA3' will terminate in a T.

Answer 1

Answer:

The correct answer is "True"

Explanation:

PCR groundwork's are short bits of single-stranded DNA, typically around 20 nucleotide long. These groundwork's ought to be reciprocal to the nucleotide groupings toward the start and the finish of the area of DNA we need to intensify and ought not corresponding to one another, else they will temper to one another and structure a "preliminary dimer".  

According to given arrangement in the information, since groundwork's are constantly determined from 5' to 3' . . ..  

4 base long arrangement piece 5'AT3' will end in G , though 4 base long succession section 5'GA3' will end in T.