Answer:
Option-2
Explanation:
Endosymbiosis theory explains the origin of the eukaryotic organism from the prokaryotes by observing the mitochondria and Chloroplast as evidence.
The endosymbiosis theory proposed that a protoeukaryote engulfed the autotrophic bacteria which evolved into the chloroplast and a chemotroph which evolved into the mitochondria.
These events could have taken when the autotrophic bacteria started using carbon dioxide and produced oxygen in the atmosphere that is around 541 million years ago.
When the concentration of the oxygen increased then the mitochondria would have used this toxic gas to produce energy and the chloroplast also formed a symbiotic relationship at that time.
Thus, Option-2 is correct.