Question

Which procedure below would lead to the visualization of the dynamic movements of specific proteins as they move through a single living cell? The proteins can be seen through the microscope eyepiece and the cells do NOT have to be killed for the protein to be detected.
a. pulse-chase using fluorescent dextran
b. the fusion of the green fluorescent protein gene to the protein that is to be tracked through the cell
c. the fusion of the green fluorescent protein gene to the gene encoding the protein to be tracked through the cell
d. pulse-chase using fluorescent antibodies
e. all of these are correct

Answer 1

Answer: Option B

Explanation:

The procedure in which the visualization of the protein is done inside the cell with destroying it can only be done when the protein which is to be tracked is inserted with fluorescent protein gene.

These fluorescent protein will move along with the protein to be tracked in order to see it.

The visualization of such proteins is possible in the microscopic view. In this case the the gene responsible for the green fluorescent is inserted in the protein to be visualized.