Robert Koch is credited with proving the germ theory of disease; that infectious diseases are caused by specific microorganisms. In developing a series of proofs (Koch's Postulates), he was able to determine the causative organism of anthrax was the bacteria Bacillus anthracis. Which of the following statements does not contribute to Koch's Postulates and the germ theory of disease?a. The causative organism must be evidenced in each subject presenting with the disease manifestations. b. Inoculating a healthy subject with the isolated organism must result in the reported disease manifestations. c. Each organism from a polymicrobial infection must be isolated and inoculated into a single host. d. The same species of organism must be isolated from the second inoculated individual. e. The organism must be isolated, purified and characterized from an infected individual.

Robert Koch is known as the founder of modern bacteriology as he provided various procedure and experimental setup to prove the germ theory. The Koch postulated the features that are related to the experiments and shows that disease is associated with a microbial organism.

The postulates were not defined for a condition caused by many bacteria that is polymicrobial infection as if the bacteria are inoculated in the host than the same disease will occur but it would be difficult to identify the causative agent of the disease. This is a limitation of the postulates of Koch.

Thus, Option-C is correct.