Answer:
Explanation:
Step 1 - Extract plasmid DNA from bacteria.
Step 2 - Cut this plasmid DNA with the help of restrictions enzyme so that gene of interest can be ligated in it.
Step 3 - Create gene of interest.
Step 4 - Combine gene of interest to plasmid creating recombinant DNA.
Step 5 - Grow transgenic bacteria carrying this plasmid.