A lab technician has four strains of bacteria. Although he forgot to label them, he knows that they include one F+ strain, one Hfr strain, one F' strain, and one F- strain. He observed the following results after plating these strains on different media. Which of the following plates can be used to identify the F- strain?a. minimal media with histidine, glutamate, and cysteine
b. minimal media with streptomycin
c. minimal media with leucine and streptomycin
d. complete media
e. minimal media with histidine, glutamate, cysteine, and streptomycin

Although F- Strain of bacteria require very minimal media due to the rate of their fertility factor but the minimal media must include at least one amino like leucine and antibiotic like streptomycin because these both are necessary for the proper replication process and indeed for the proper growth.

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