Question

DNA cloning Place the following steps in order to describe the process of DNA cloning. Drag the text blocks below into their correct order. Plasmids isolated from bacteria are incubated with the insulin gene and DNA ligase. A gene for insulin is removed from a human cell. 3 Bacteria are made to take up plasmids containing recombinant DNA. Bacteria produce insulin, which can be extracted, purified, and used. Recombinant DNA is created.

Answer 1

Answer:

The correct sequence of the cloning process is mentioned below:

A gene for insulin is removed from a human cell.

Plasmids isolated from bacteria are incubated with the insulin gene and DNA ligase.

Recombinant DNA is created.

Bacteria are made to take up plasmids containing recombinant DNA.

Bacteria produce insulin, which can be extracted, purified, and used.

Explanation:

The process of molecular cloning is characterized by the creation of the recombinant  DNA by placing it in the host and directing the replication process to get the desired result. The word cloning is justified because of the formation of identical DNA strands(gene of interest). Some of its major application includes the study of genome organisation and gene expression, formation of transgenic organism and recombinant protein and gene therapy.

Answer 2

The correct order for gene cloning in this case is:

1. First step:- Gene for insulin is removed from the human cell.  
2. Second step:- Plasmids isolated from bacteria are incubated with the insulin gene and DNA ligase.  
3. Third step:- Recombinant DNA is created.  
4. Fourth step:- bacteria are made to take up plasmids containing recombinant DNA.  
5. Fifth step:- Bacteria produce insulin which can be excreted, purified, and used.

The human insulin gene is transferred into a bacterial cell to produce a large amount of insulin, to administrated to patient's deficiency of insulin in his or her body.

Gene cloning for insulin production has the following steps:

1. First step:- Gene for insulin is removed from the human cell.  
2. Second step:- Plasmids isolated from bacteria are incubated with the insulin gene and DNA ligase.  
3. Third step:- Recombinant DNA is created.  
4. Fourth step:- bacteria are made to take up plasmids containing recombinant DNA.  
5. Fifth step:- Bacteria produce insulin which can be excreted, purified, and used.

Thus, the correct order for gene cloning in this case is:

1. First step:- Gene for insulin is removed from the human cell.  
2. Second step:- Plasmids isolated from bacteria are incubated with the insulin gene and DNA ligase.  
3. Third step:- Recombinant DNA is created.  
4. Fourth step:- bacteria are made to take up plasmids containing recombinant DNA.  
5. Fifth step:- Bacteria produce insulin which can be excreted, purified, and used.

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