Question

You aseptically transfer 1 mL of your original liquid culture into 99 mL of sterile water. You then repeat this procedure two more times. What is the final dilution factor of this serial dilution? 1. 10^-4. 2. 10^2. 3. 10^-6.4. 10^-2. 5. 10^2

Answer 1

Answer is option "1"

Explanation:

• A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step.  
• In serial dilution, on the off chance that you are diluting to a similar extent, you duplicate the weakening components for each progression. The dilution or dilution factor ($D_{f}$) is the underlying volume ($V_{i}$) separated by the last volume ($V_{f}$) for example:
• 1 ml of liquid trnasfer in 99 ml of sterile water to complete the solution of 100 ml.
• formula of serial dilution is ∴ $D_{f}$ = $\frac{V_{i} }{V_{f} }$
• The solution repeat two times,  exact serial dilution is $10^{-2}$ ml then total dilution is  $10^{-4}$
• Hence, the right answer is option 1 "$10^{-4}$ ".