Answer:correct answer is E. may correlate with an organism's ability to cause disease,are typically negatively stained and are stained as wet mount.
Explanation:
Capsule is a hydrated polysaccharide structure that cover the outer layer of the bacterial cell wall.It is considered as a virulence factor because it enhances the ability of the bacteria to cause disease.
Because capsules are tightly packed,they cannot be stained with standard stains as the capsule cannot absorb these dyes .So in such cases,negative staining technique is employed where the bacteria cells and its background are stained darker than the capsule(which doesn't stain).By this method,capsule can easily seen under microscope.