Question

For each of these molecules, calculate how much ATP could be produced under the following conditions; if there is net ATP consumption, then use a negative number. Unless otherwise indicated, you may use any of the enzymes covered in the pathways covered in the first part of the exam (i.e. assume normal glycolysis and TCA cycle). (4.5 points each)

Answer 1

In Glycolysis 2ATP is produced

In TCA cycle 12 ATP is produced

Explanation:

In Glycolysis total there are 4 ATPs produced 2 molecules of ATP is used in the phosphorylation of glucose molecule and in the formation of glucose 1,6 bisphosphate. This phase of Glycolysis is called as Pay Off phase.

Glycolysis converts 1 molecule of glucose into 2 Pyruvate molecules, 4ATP and 2 NADH. From which 4ATP-2ATP.

In KCA Cycle:

KCA consists of 8 steps reactions, during the cycle only 1 GTP=1ATP and 3 NADH and 1 FADH2. The redox cofactors will generate ATP in ETC.

1 NADH= 3 ATP

1 FADH2=2ATP

so, practically if considering the future of these cofactors 12 ATPs are produced however TCA undergoes two cycles per glucose molecule.