Answer:
The sample size of the animal was too small , not large enough to conclude that the effects of the chemicals on Salamander was the actual cause of toxicity and weight loss.
It is possible that the drop in weight may be as a result of other environmental factors; agitation from background noise and sudden exposure to new environments,, fluctuation in temperature of the surrounding environments , lack of feed supply or others
However, if there was many salamanders available; and most reacted to the chemical exposure in the same way then it can be concluded that, toxicity of the chemical is the causative factor.
Explanation:
