Answer:
1. Choose desired DNA and plasmid vector.
2. Cut the desired DNA fragment and plasmid with the same restriction enzyme: The second step in producing recombinant DNA is getting the desired DNA fragment by cutting it with a specific restriction enzyme.
The vector which is used to transfer this desired DNA fragment in host should also be cut by the same restriction enzyme to produce sticky ends.
3. Ligation: Then the desired DNA fragment should be ligated in the plasmid vector by the help of the ligase enzyme.
4. Transformation of bacteria: Now the recombinant DNA molecule should be injected into bacteria by using some technique like electroporation, heat shock method, etc.
