A
The first step in the hybridization of radioactive DNA and normal DNA is to Heat a mixture of the two types of DNA.
Explanation:
This first step allows the double-stranded DNA to separate into single strands of DNA. The heating breaks the H-bonds between the nucleotides bases of complementary DNA strands. After heating, the solution will, therefore, have a homogenous mixture of radiolabeled and normal single-stranded DNA. Usually, this temperature is approximately 95 degrees centigrade but varies around depending on a number of C-G and A-T bonds in the dsDNA.
The following step is to lower down the temperatures so the single-stranded DNA can re-anneal. The resulting dsDNA in the solution will have a proportion of those with a normal strand and radiolabeled complementary strand annealed.
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