You isolate intact mitochondria and equilibrate them in a buffered solution at pH 9, containing 0.1 M KCl and ADP plus Pi, but without succinate. You collect them by centrifugation, and quickly resuspend them in a new buffer at pH 7, without KCl , but with valinomycin (a K ionophore). Note: the K rushing out will create a huge positive charge differential. What do you predict will be the result on oxygen consumption and the production of ATP?
