Which of the following are characteristics of reverse-phase chromatography?I The stationary phase is polar.II The mobile phase is more polar than the stationary phase.III Less polar mobile phase has a lower eluent strength.IV More polar mobile phase has a higher eluent strength.V The stationary phase is nonpolar.

The characteristics of reversed phase chromatography are The mobile phase is more polar than the stationary phase and The stationary phase is non polar.

Hence, option II and V are correct.

What is RPC?

RPC is Reversed Phase Chromatography. In reversed phase chromatography the stationary phase is non polar [hydrophobic] and the mobile phase is very polar [hydrophilic].

Now lets check all the option one by one:

Option (I): The stationary phase is non polar in reverse phase chromatography.

So it is incorrect option.

Option (II): The mobile phase is more polar than the stationary phase because in polar mobile phase has high affinity towards the polar solute.

So, it is correct option.

Option (III): Less polar mobile phase has higher eluent strength not lower eluent strength.

So, it is incorrect option.

Option (IV): More polar mobile phase has less eluent strength.

So, it is incorrect option.

Option (V): The stationary phase is non polar [hydrophobic] is reverse phase chromatography.

So, it is correct option.

Thus, from above conclusion we can say that the The mobile phase is more polar than the stationary phase and The stationary phase is non polar are the characteristics of reverse phase chromatography.

Hence option II and V are correct.

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