Question

What can be said about all the DNA molecules in a single Sanger sequencing reaction mix? Select All That Apply
DNA can be sequenced less expensively.
Fewer errors in the sequence are produced.
A whole-genome could be sequenced in a single day.
DNA can be sequenced much faster.

Answer 1

Answer:

Fewer errors in the sequence are produced.

Explanation:

Sanger sequencing is the oldest sequencing methodology developed by Frederick Sanger in 1977. The technique is still in practice today for sequencing of single genes to achieve high accuracy of DNA strands sequencing  compared to modern sequencing technologies such as Next Generation Sequencing (NGS) platforms (Illumina Sequencing).

Among the given statements, only B is correct because

1. Sanger sequencing is costly compared to NGS technologies (per kb cost for NGS is 20 cents whereas for Sanger sequencing it is about $10).
2. Sanger sequencing is not a technology for whole genome sequencing (WGS) rather for single-gene sequencing with high accuracy. For WGS, massively parallel sequencing is required which can be achieved with NGS platforms.