Question

To create a molecule of recombinant DNA, which of the following is cut with a restriction enzyme? A molecule of double stranded recombinant DNA including a part of foreign DNA. Check all that apply.
1. target DNA
2. target RNA
3. DNA ligase
4. starting DNA

Answer 1

Answer:

1. Target DNA

4. Starting DNA

Explanation:

A recombinant DNA molecule has a sequence that is obtained or inserted from a different organism. A target DNA is the one that obtains a foreign gene sequence. The DNA molecule that serves as a donor of the gene sequence is called starting DNA. To create recombinant DNA molecule, the target DNA and the starting DNA are cut with a restriction enzyme. Restriction enzymes cut the DNA at specific restriction sites. Digestion of target DNA and starting DNA with restriction enzyme creates DNA fragments with complementary ends. It facilitates their ligation.