Question

The major advantage of using artificial chromosomes such as YACs and BACs for cloning genes is that

plasmids are unable to replicate in cells.
only one copy of a plasmid can be present in any given cell, whereas many copies of a YAC or BAC can coexist in a single cell.
YACs and BACs can carry much larger DNA fragments than ordinary plasmids can.
YACs and BACs can be used to express proteins encoded by inserted genes, but plasmids cannot.
all of the above
H. Simply inserting an entire eukaryotic gene into a prokaryotic expression system will most likely not work for the following reason.

Prokaryotes lack promotors
Prokaryotes lack introns
Prokaryotes lack polymerase
Prokaryotes lack repressors

Answer 1

Question is a multiple choice question.

Answer:

Question 1. The major advantage of using artificial chromosomes such as YACs and BACs for cloning genes is that:

Answer:

(C)

Explanation:

YACs and BACs can carry much larger DNA fragments than ordinary plasmids can.

Question 2. Simply inserting an entire eukaryotic gene into a prokaryotic expression system will most likely not work for the following reason.

Answer:

(B)

Explanation:

Prokaryotes lack introns