Question

The following are four processes common to most cloning experiments: i) transforming bacteria ii) plating bacteria on selective medium iii) cutting DNA with restriction endonucleases iv) ligating DNA fragments Place the components of this list in the order in which they would most likely occur during a cloning experiment.

Answer 1

Answer:

The correct answer will be-

1. Cutting DNA with restriction endonucleases

2. Ligating the DNA fragments with DNA ligase

3. Transforming bacteria

4. Plating bacteria

Explanation:

DNA cloning is the widely used technique which makes exact copies of the specific piece of DNA by incorporating the DNA into bacterial chromosome mainly plasmid.

The technique of cloning the DNA into bacteria is known as bacterial transformation which is performed following the protocol:

1. Cutting DNA with restriction endonucleases and inserting the gene of interest.  

2. Ligating the DNA fragments with DNA ligase which ligates or joins the selected piece of the enzyme to the plasmid after incorporation.

3. Transforming bacteria by growing the bacteria into a solution containing the plasmid. The bacteria are killed by heat which allows the bacteria to uptake plasmid.

4. These bacteria are then grown into the selective media by the plating method.