Question

To clone the entire coding region of p53 into the plasmid, which restriction enzyme(s) could you use to cut both your PCR product and the pCDNA3 plasmid? Select all that apply. Select all that apply. E (EcoRI) B (BamHI) H (HindIII) S (SmaI) X (XbaI) N (NotI)

Answer 1

Answer:

E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI).

Explanation:

Only SmaI does not have a recognition site in the polylinker of pCDNA vector (See map attached). So, in theory, any of the other enzymes:  E (EcoRI) B (BamHI) H (HindIII) X (XbaI) N (NotI) could be used. It would be of importance to see in silico the digestion of regions flanking the p53 gene.