Question

What is the best explanation if the gel of your PCR product shows smeared bands of multiple sizes? Select one:
a. Non-specific gene amplification
b. Primers hybridizing with one another
c. Your isolate was not from a single bacterial colony
d. All of the above
e. None of the above

Answer 1

Answer:

e. None of the above

Explanation:

For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA  which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.  

i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.