How might you determine how much peroxidase is ACTUALLY in the volume of extract used to create your linear curve? Think about the experiments you might need to do. Refer to your biochemistry textbook if you need ideas.

An evaluation of the impact of immunoenzymatic assays on immunochemical-based analytics in the last 4 decades, in the detection of infectious agents or products associated with their presence and (or) pathogenic activity was made. In addition, there is an incursion into some designs and formats that these immunoassays have had since electrochemical detection methods, tests to detect proteolytic activity of microbial origin and its inhibitors as possible therapeutic targets, direct triple antibody immunoassays to achieve greater sensitivity, alternative developers of enzymatic activity, trials for the study of viral serology with a minimum of determinations, as well as proficiency tests to evaluate the efficacy of controlled vaccine candidates in selected peptide combinations. It was concluded with a quick vision of the immediate future of this type of immunoassays in the light of emerging analytical detection technologies.