An Environmental Police Officer would like to determine if a duck that was harvested by a hunter was a black duck, or a hybrid between a black and mallard duck. She would like to compare the DNA from the hunter’s duck to a pure black duck and a pure mallard. So that there will be enough DNA to analyze, which biotechnology technique will she use to increase the amount of DNA in the samples?A. Gel electrophoresisB. Therapeutic cloningC. Polymerase chain reactionD. Recombinant DNA techniquesE. None of the above.

In the first step of this reaction, a double stranded DNA molecule is denatured using high temperature and the strands are separated. Then, the single strands of DNA are hybridized with a short fragment of DNA called a primer, which is complementary to a short sequence of the template DNA. The primers are then elongated by the DNA polymerase, an enzyme that adds nucleotides complementary to the template strand. As a result two double stranded DNA molecules identical to the original are generated. By repeating these steps many times (typically between 15 and 30), you can obtain millions of DNA molecules with the sequence that you wish to analyze